Intracellular distribution and axonal transport of PKD in neurons

Abstract

Event Abstract Back to Event Intracellular distribution and axonal transport of PKD in neurons T. Szórádi1*, F. Klacsmann1, F. Vajda1, K. Tárnok1 and K. Schlett1 1 Eötvös Loránd University, Department of Physiology and Neurobiology, Hungary Protein Kinase D (PKD) is a serine/threonine kinase, known to regulate dendritic maintenance and secretory transport in neurons. PKD-regulated axonal events, however, are not identified yet. In this study we quantitatively examined the intracellular distribution of EGFP-tagged wild type, point or deletion mutants of PKD in transfected primary hippocampal neurons. While the majority of the investigated mutants were evenly distributed, dominant-negative kinase-inactive form of PKD1 or a construct lacking the kinase domain were found to be excluded from the axons and restricted to the somatodendritic compartment. Similar uneven intracellular localization was observed when PKD1 mutants lacking the PH (Pleckstrin homology) domain or amino acids 80-146 were used. These findings suggested that beside kinase activity, the N terminal region also regulates PKD’s axonal presence. In order to identify the potential transport differences responsible for the altered intracellular distribution of the mutant PKD proteins, live cell recordings were made from transfected axonal segments. In several cases, vesicle-like transportation of EGFP-tagged PKD mutants was observed. Detailed analysis of the dynamic properties (e.g. cumulative distance, straight distance, mean velocity) of the fluorescent vesicle-like structures is in progress and will help to clarify the regulation of PKD’s intracellular distribution. This work will help to elucidate the potential role of PKD in axons, as well. Keywords: Molecular and cellular neurobiology, Neuroscience Conference: 13th Conference of the Hungarian Neuroscience Society (MITT), Budapest, Hungary, 20 Jan - 22 Jan, 2011. Presentation Type: Abstract Topic: Molecular and cellular neurobiology Citation: Szórádi T, Klacsmann F, Vajda F, Tárnok K and Schlett K (2011). Intracellular distribution and axonal transport of PKD in neurons. Front. Neurosci. Conference Abstract: 13th Conference of the Hungarian Neuroscience Society (MITT). doi: 10.3389/conf.fnins.2011.84.00049 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 03 Mar 2011; Published Online: 23 Mar 2011. * Correspondence: Dr. T. Szórádi, Eötvös Loránd University, Department of Physiology and Neurobiology, Budapest, Hungary, szoradi.tamas@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers T. Szórádi F. Klacsmann F. Vajda K. Tárnok K. Schlett Google T. Szórádi F. Klacsmann F. Vajda K. Tárnok K. Schlett Google Scholar T. Szórádi F. Klacsmann F. Vajda K. Tárnok K. Schlett PubMed T. Szórádi F. Klacsmann F. Vajda K. Tárnok K. Schlett Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.