Intracellular Ca 2+ does not activate the SITS-sensitive anion transporter in barnacle muscle

Abstract

By means of the intracellular dialysis technique, we have measured 36Cl efflux from single barnacle muscle fibers and compared the effects of raising intracellular ionized calcium concentration ([Ca 2+] i) to the effects of lowering intracellular pH (pH i). Lowering pH i by 1 unit or less resulted in a 20-fold stimulation of 36Cl efflux which occurred relatively rapidly and which could be inhibited by 90–95% by 4-acetamido-4′-isothiocyanostilbene-2,2′-disulfonic acid (SITS). In contrast, raising [Ca 2] i as much as 250-fold resulted in a relatively small increase of 36Cl efflux. The small increase occurred after a long latency, developed slowly and could not be blocked or prevented by treatment with SITS. We conclude that the increase of the SITS-sensitive 36Cl efflux caused by a fall of pH i is not mediated by a rise of [Ca 2+] i.