Abstract
1. 1. Goldfish were acclimated to temperatures ranging from 21.5 to 1°C. 2. 2. Acclimation to near freezing temperatures caused a reduction in plasma osmolality due to decreased organic solutes as well as reduced plasma inorganic ions. 3. 3. Plasma Na + concn decreased in lower temperatures; plasma Cl − did not. 4. 4. RBC response to decreased plasma osmolality included phases: osmotic swelling followed by cellular shrinkage. 5. 5. Cellular shrinkage resulted from efflux of KC1 and water. 6. 6. The over-all response is an example of “isosmotic intracellular regulation” achieved by adjusting osmotically active particles.
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