Abstract
Prostaglandin F 2α (PGF 2α) is a major stable prostaglandin formed in vivo in physiological and pathophysiological situations and has mainly potent vasoconstrictive and pro-inflammatory properties. PGF 2α is now used as an indicator of acute and chronic inflammation in human clinical settings but the extent of daily variation of PGF 2α in vivo in healthy humans is unknown. We quantified levels of the PGF 2α metabolite 15-keto-dihydro-PGF 2α in 10 healthy males and females in spot urine samples during the day (including morning urine sample) and in 24-h urine during the same day. The intra-day coefficient of variation was 20.9%. However, the total mean value of 15-keto-dihydro-PGF 2α in urine collected in the morning did not significantly differ from the mean level of 15-keto-dihydro-PGF 2α in the 24-h urine samples in the 10 subjects. 15-Keto-dihydro-PGF 2α levels in morning urine showed a positive linear correlation with levels of 15-keto-dihydro-PGF 2α in 24-h urine ( R = 0.72, P < 0.05). In conclusion, formation of PGF 2α shows a biological variation within the day in healthy humans which should not be overlooked when planning a clinical study. Single morning urine samples can be used as an alternative to 24-h urine collections for quantification of PGF 2α formation to simplify the sampling regime in larger clinical studies.
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