Intestine of invasive fish Prussian carp as a target organ in metal exposure assessment of the wastewater impacted freshwater ecosystem

Tatjana Mijošek,Vlatka Filipović Marijić,Zrinka Dragun,Dušica Ivanković,Nesrete Krasnići,Zuzana Redžović,Marijana Erk

doi:10.1016/j.ecolind.2020.107247

Tatjana Mijošek, Vlatka Filipović Marijić + Show 5 more

Open Access

https://doi.org/10.1016/j.ecolind.2020.107247

Copy DOI

Journal: Ecological Indicators	Publication Date: Dec 22, 2020
Citations: 3	License type: cc-by

Affiliation: Rudjer Boskovic Institute

Abstract

The application of invasive fish Prussian carp (Carassius gibelio Bloch, 1782) as bioindicator organism, using intestine as bioindicator tissue of anthropogenic influence in the lowland Ilova River was estimated. Intestinal tissue enables the investigation of dietborne metal uptake, so the first record on intestinal metal levels in Prussian carp was presented, as total and cytosolic fraction, which indicates the proportions of potentially toxic and bioavailable metals. Pollution impact was also estimated by analyses of biomarkers of oxidative stress (malondialdehyde), antioxidative capacity (catalase and glutathione) and of metal exposure (metallothioneins). All analyzed parameters were compared in the intestine of fish from the reference site and contaminated site impacted by technological and municipal wastewaters in two seasons. Both total and cytosolic As, Ca, Cd, Cs, Cu, Mg, Na and Rb levels were significantly higher at contaminated than the reference site in at least one season, whereas Mn and V had higher concentrations at the reference site. Despite differences in concentrations, average proportions of total metal levels in cytosolic fraction were comparable at two sites, i.e. over 70% for Na, K, Rb, Se, Cd, Cs, As and Mo, indicating their high possibility of binding to important biomolecules. In addition, higher levels of malondialdehyde in both seasons and enhanced catalase activity in spring, indicated disturbed environmental conditions near the contaminated site and need of continuous monitoring of this region. Finally, our research represents successful application of widely distributed invasive species in ecotoxicological studies, whereas intestine was shown as a suitable bioindicator tissue, clearly reflecting dietary metal uptake.

Highlights

Prussian carp (Carassius gibelio Bloch, 1782), a cyprinid fish species nowadays widely distributed in Europe and Asia, has a high invasion potential and can tolerate unfavorable environmental conditions including low oxygen levels, variable temperatures and high levels of anthropogenic pollution (De Boeck et al, 2004)
Spatial differences were evident as higher values of all biometric parameters in fish from the Trebez village compared to the reference site, except of comparable HSI levels in autumn
Prussian carp itself was shown as a suitable bioindicator species in aquatic environmental pollution assessment and the intestine as suitable bioindicator organ, which reflects responses to contaminants from di etary pathways

Summary

Introduction

Prussian carp (Carassius gibelio Bloch, 1782), a cyprinid fish species nowadays widely distributed in Europe and Asia, has a high invasion potential and can tolerate unfavorable environmental conditions including low oxygen levels, variable temperatures and high levels of anthropogenic pollution (De Boeck et al, 2004). It is an appropriate bioindicator in pollution assessment studies (De Boeck et al, 2004; Falfushynska et al, 2011; Tsangaris et al, 2011), including metal contamination. Metals in cytosolic fraction can bind and interact with either biologically available part containing microsomes and heat sensitive proteins (e.g., enzymes) or detoxified part involving heat-stable proteins (e.g., metallothioneins) (Bonneris et al, 2005; Urien et al, 2018)

Objectives

Methods

Results

Discussion

Conclusion