Abstract
Abstract A feeding experiment with purified diets was conducted in a controlled environment to study intestinal synthesis and dietary essentiality of vitamin B12 in Tilapia nilotica. Intestinal microorganisms in T. nilotica fed a vitamin B12-deficient diet synthesized at least 11.2 ng of vitamin B12 per g of body weight per day. Removal of cobalt from the diet or supplementation with an antibiotic (succinylsulfathiazole) significantly reduced the rate of intestinal vitamin synthesis. Dietary supplementation of vitamin B12 was not necessary for normal growth and erythropoiesis or to prevent a decrease in liver-stored vitamin B12 over a 16-week feeding period during which fish weight increased eight-fold.
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