Abstract
Long non-coding RNAs (lncRNAs) are important in regulating the response to environmental stresses in organisms. In this study, we used Caenorhabditis elegans as an animal model to determine the functions of intestinal lncRNAs in regulating response to simulated microgravity stress. Among the intestinal lncRNAs, linc-2, linc-46, linc-61, and linc-78 were increased by simulated microgravity treatment, and linc-13, linc-14, linc-50, and linc-125 were decreased by simulated microgravity treatment. Among these 8 intestinal lncRNAs, RNAi knockdown of linc-2 or linc-61 induced a susceptibility to toxicity of simulated microgravity, whereas RNAi knockdown of linc-13, linc-14, or linc-50 induced a resistance to toxicity of simulated microgravity. In simulated microgravity treated nematodes, linc-50 potentially binds to three transcriptional factors (DAF-16, SKN-1, and HLH-30). RNAi knockdown of daf-16, skn-1, or hlh-30 could suppress resistance of linc-50(RNAi) nematodes to the toxicity of simulated microgravity. Therefore, our results provide an important basis for intestinal lncRNAs, such as the linc-50, in regulating the response to simulated microgravity in nematodes.
Highlights
RNAs in response to simulated microgravity stress in Caenorhabditis elegans Lingmei Sun, Dan Li, Yujie Yuan & Dayong Wang*
Among the intestinal Long non-coding RNAs (lncRNAs), linc-2, linc-46, linc-61, and linc-78 were increased by simulated microgravity treatment, and linc-13, linc-14, linc-50, and linc-125 were decreased by simulated microgravity treatment
We first investigated the effects of simulated microgravity treatment for 4, 8, 12, or 24 h on two aspects, induction of reactive oxygen species (ROS) production and locomotion behavior
Summary
RNAs in response to simulated microgravity stress in Caenorhabditis elegans Lingmei Sun, Dan Li, Yujie Yuan & Dayong Wang*. Long non-coding RNAs (lncRNAs) are important in regulating the response to environmental stresses in organisms. We used Caenorhabditis elegans as an animal model to determine the functions of intestinal lncRNAs in regulating response to simulated microgravity stress. RNAi knockdown of daf-16, skn-1, or hlh-30 could suppress resistance of linc-50(RNAi) nematodes to the toxicity of simulated microgravity. Our results provide an important basis for intestinal lncRNAs, such as the linc-50, in regulating the response to simulated microgravity in nematodes. Treatment with simulated microgravity could inhibit locomotion behavior, induce intestinal damage, and activate oxidative stress reflected by reactive oxygen species (ROS) production[18,19,20]. We focused on the identification of intestinal lncRNAs involved in control of response to simulated microgravity. We determined the underlying mechanism for linc-50, one of the identified lncRNAs, in regulating response to simulated microgravity
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