Intestinal Anti-Inflammatory and Anti-Oxidant Activity of The Aqueous Extract From Arum Dioscoridis in Acetic Acid Induced Colitis in Rats

Abstract

Objective Arum dioscoridis is a plant that has been shown to regulate biological activity on oxidation, immune system and inflammation grown in our country. We aimed to investigate the effects of arum dioscoridis in experimental colitis related to acetic acid in rats. Material and Methods In this study 56 Wistar Albino female rats, 6 weeks old, weighing 180- 210 g, were seperated into 7 equal groups with 8 animals in each group. Group 1 is the control one without any administration. An experimental colitis has been created on Group 2. Group 3 is treated with prophylactic oral arum dioscoridis before creating experimental colitis. The rats belongs to Group 4 has been received oral arum dioscoridis treatment with ethanolic solvent after creating experimental colitis. Group 5 was given oral treatment with solvent with DMSO (Dimethyl sulfoxide) after experimental colitis was created. Group 6 rectal treatment with solvent with DMSO after experimental colitis has been created. Group 7 was given a 2-fold oral treatment after experimental colitis. In tissue samples taken from mice, the effects of arum dioscoridis were evaluated macroscopically, histopathologically and biochemically. Results In the colitis group, significant weight loss occurred compared to the treatment groups. Statistically significant improvement was found in macroscopic and microscopic analysis with arum dioscoridis treatment and prophylaxis. In the colitis model which is created with acetic acid, total oxidant status, thiol / disulfide, malondialdehyde, myeloperoxidase levels increased and a decrease in total antioxidant capacity was detected. Conclusion This study showed that arum dioscoridis has positive effects on the colitis model created in rats, and it significantly improves intestinal inflammation especially in groups given by dissolving with ethanol and used for prophylaxis purposes