Interspecific Repellent Effect of Hydroalcoholic Solution of Nest Refuse from Two Leaf-cutter Ant Species

Abstract

Leaf-cutting ants manage their waste continuously to avoid risks of colony contamination. The presence of residues or their odor near foraging areas is enough for ants to stop attacking plant tissues. However, to date, no study has demonstrated an interspecific effect of nest waste on the deterrence of leaf-cutting ants. Here, we tested whether a hydroalcoholic extract of nest refuse (NR) of the leaf-cutting ants Acromyrmex balzani Emery, 1890 and Atta opaciceps Borgmeier (Hymenoptera: Formicidae, Attini) causes an interspecific deterrent effect. Field colonies of Ac. balzani (n = 30) and A. opaciceps (n = 36) were used. For each colony, we offered baits made with plastic straws covered by citrus pulp and sprayed with a hydroalcoholic solution of 20% (vol/vol) alcohol/water (Control) or an extract of 20% (vol/vol) of NR from Ac. balzani and/or A. opaciceps. Over 20-30 minutes we recorded the amount of baits removed and data were compared through survival analysis and Linear Mixed Effect (LME) model. Ants avoided collecting baits sprayed with NR resulting in less than 20% of these baits being carried to the nest versus 80% of control baits. The deterrence was both intra and interspecific, considering that the response was similar for baits from both species. Such results reinforce the premise that the presence of nest refuse alters the foraging behavior of ants and paves the way for new studies that can verify its effectiveness in reducing foliar consumption on a larger spatial scale.