Interspecific relationships in Fagopyrum (Polygonaceae) revealed by the nucleotide sequences of the rbcl and accd genes and their intergenic region

Abstract

DNA sequences of the rbcL and accD 5' coding regions and their intergenic region (IGR) were determined in 20 taxa of 12 species of Fagopyrum. By comparing the sequence data and constructing phylogenetic trees, phylogenetic relationships among Fagopyrum species were investigated. The accD 5' coding region and the IGR have evolved about five times faster than the rcbL coding region; hence the phylogenetic trees based on the data of the accD 5' coding region, the IGR, and these data combined, had higher resolution than the trees based on rcbL alone. The classification of Fagopyrum based on the DNA sequences was almost perfectly consistent with the classification by Ohnishi and Matsuoka (1996, Genes & Genetic Systems 71: 383-390). New facts and minor differences are (1) polyploidization in F. cymosum might have occurred more than once, (2) F. lineare may not be a sister species of F. leptopodum, and (3) F. pleioramosum rather than F. capillatum may be a sister species of F. gracilipes.