Abstract
Summary Certain crosses of Arachis hypogaea × A. stenosperma did not produce viable F 1 hybrid seed when pods were left on the plant to mature. The cause of embryo abortion is unknown but probably was related to incompatibility between hybrid embryo and maternal tissues that may have resulted in nutritional deficiency. Viable hybrid embryos were rescued using three protocols: 1) in vitro embryo maturation and germination; 2) culture of shoot-root embryo axis attached to a single cotyledon in a cytokinin-rich medium; and 3) production of somatic embryos from the excised cotyledon. The rescue of viable hybrid embryos was accomplished with nearly 100 % efficiency using protocol 1. Both protocols 2 and 3 were found to be less efficient methods but may have advantages over protocol 1 under certain circumstances. Frequency of somatic embryogenesis appeared to be genotype dependent. Several interspecific hybrid plants were recovered with all three methods.
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