Abstract

The lepidopteran pupae of Aglais urticae L., Inachis io L. (Nymphalidae), and Pieris brassicae L. (Pieridae) show a morphological colour adaptation. Their melanization is controlled by a pupal melanization reducing factor, which gradually inhibits cuticular melanization. Extracts from head-prothorax fragments of A. urticae as well as of Galleria mellonella L. (Pyralidae), a species without a pupal colour adaptation, reduce melanization in pupae of I. io in a dose-dependent way. However, extracts of P. brassicae injected into I. io show a weaker melanization reducing factor activity than those of the other two species. Pupal melanization reducing factor extracts from head-prothorax fragments of Inachis is show dose-dependent melanization reducing effects on the pupal colouration of all three species, A. urticae, I. io, and P. brassicae.

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