Abstract
Discontinuous polyacrylamide gel electrophoreses for lactate dehydrogenase (Ldh) and alfa-esterase (α-Est) isoenzymes were conducted for biochemical differentiation between Echis pyramidum and Eryx colubrinus inhabiting El-Faiyum, Egypt. Total lipids and total protein of liver and muscle tissues in both species were also analyzed. Three Ldh isoforms were recorded in both species and the activity and relative front (RF) of Ldh-1 seemed to be higher in E. pyramidum than in E. colubrinus. This high activity could be supported by the significant increase in the total lipids and total protein in liver and muscle tissues of this species. It is thus possibly reasonable to suppose that E. pyramidum is more active, energetic and adaptable in its habitat than E. colubrinus. α-Est showed five isozymic forms fractions in E. pyramidum, while it showed only four isoforms in E. colubrinus. α-Est-1 was the first clear, dense and thick isoform in E. pyramidum, but it was completely absent in E. colubrinus. High activity of the esterase isoform; α-Est-1 only in the heart tissue of E. pyramidum may reflect the high ability of E. pyramidum to be more resistant to the accumulated toxic residues in its body tissues than E. colubrinus.
Highlights
The squamates are the most diverse group containing the lizards and snakes [1]
Our present study aimed to investigate the patterns of inter-specific biochemical and genetic diversity between Echis pyramidum and Eryx colubrinus inhabiting two different sites in El-Faiyum desert of Egypt using the electrophoretic analyses of two isoenzymes
It is possibly reasonable to suppose that E. pyramidum is more active, energetic and adaptable in the desert habitat than E. colubrinus
Summary
The squamates are the most diverse group containing the lizards and snakes [1]. Several investigations haveHow to cite this paper: Kadry, M.A.M., Mohallal, E.M.E., Sleem, D.M.M. and Marie, M.A.S. (2015) Inter-Specific Biochemical Diversity between Echis pyramidum and Eryx colubrinus Inhabiting El-Faiyum, Egypt. The squamates are the most diverse group containing the lizards and snakes [1]. How to cite this paper: Kadry, M.A.M., Mohallal, E.M.E., Sleem, D.M.M. and Marie, M.A.S. (2015) Inter-Specific Biochemical Diversity between Echis pyramidum and Eryx colubrinus Inhabiting El-Faiyum, Egypt. Advances in Bioscience and Biotechnology, 6, 495-500. The suborder Serpentes is distributed all over the world [4] [5]. Previous descriptions of the external and taxonomical features of some snakes have been ambiguous and unreliable. Several authors used the biochemical electrophoresis [6] and molecular sequence analysis [3] [7]-[13] to resolve the cladistic relationships among snakes and to clarify their phylogeny
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