Interspecies comparison of temporomandibular joint condylar cartilage extracellular matrix from macro to microscopy

Abstract

Interspecies comparisons of the extracellular matrix of temporomandibular joint (TMJ) condylar cartilage are necessary to elucidate the mechanisms underlying its superior mechanical properties, to guide the construction of animal models of TMJ-related diseases, and to establish standards for the engineering of TMJ condylar cartilage. Here we characterize and compare TMJ condylar cartilage from six different species from a materials science perspective, including structure, composition and mechanical properties from the macroscopic to the microscopic level. The gross morphology showed obvious interspecies differences in size and shape, which may be related to the different joint motion patterns. Although the condylar cartilage of all species can be divided histologically into a superficial fibrous layer and a deep hyaline layer, there are significant interspecies differences in the microstructure of the fibrils in the two layers, mainly in the diameter of the fibrils. Compositionally, there were no significant differences in collagen composition between species, but the content of glycosaminoglycans (GAGs) decreased progressively with increasing body size, with the same results obtained by Safranin O staining and biochemical analysis. Mechanically, the elastic modulus of mouse condylar cartilage was significantly higher than that of the other species and tended to decrease with increasing body size. This study shows that the TMJ condylar cartilage of different species has its own specific structure-composition-mechanics matching characteristics for their unique masticatory stress dissipation, and differences in fibril diameter and GAGs content may be the two ultimate factors influencing the differences in cartilage mechanical properties between species, while the condylar cartilage of pigs is most similar to that of humans, suggesting that pigs may be a suitable animal model for TMJ studies.