Abstract
Abstract Recent studies suggest that a linear correlation exists between the age of individual birds and accumulation of the advanced glycation endproduct pento- sidine (Ps). We compared Ps accumulation in skin tissue of Ruffed Grouse (Bonasa umbellus) and Double-crested Cormorants (Phalacrocorax auritus). Skin samples were taken from the breasts of 52 Ruffed Grouse of known ages ranging from a few days to 10 years. Skin was cleaned of feathers and adipose tissue, acid hydrolyzed, and measured for collagen content via hydroxyproline spectrophotometric assay. Pentosidine concentrations were determined using reverse-phase high-performance liquid chromatography. Fluorescent detection (excitation: 310 nm, emission: 385 nm) revealed that concentrations of Ps per milligram of collagen increased curvilinearly with age (r2 = 0.87, P < 0.0001). Additionally, a blind study of 19 Double-crested Cormorants revealed a linear relationship between Ps concentrations and age (r2 = 0.93). Comparison of these two species re...
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