Abstract
1. 1. Ehrlich ascites carcinoma cells grown for 7–8 days in C 57/BL mice were used for in vitro chemical and metabolic time studies to evaluate the inhibition of oxygen consumption by the cell (Crabtree Effect). 2. 2. The time of onset of inhibited respiration was measured with an oxygen electrode. 3. 3. Determinations were made of the influence of monoiodoacetic acid and 2,4-dinitrophenol on the respiration of the tumor cells endogenously and in the presence of glucose. 4. 4. The rates of glucose and 2-desoxy- d-glucose metabolism and changes in certain metabolites (lactate, trioses, ketoses, pentose, ATP, ADP, AMP, DPN, TPN and total mono- and dinucleotides) were correlated with the onset, duration and termination of the Crabtree Effect. 5. 5. The influence of 2,4-dinitrophenol on glucose metabolism and changes in the same metabolites as indicated in point 4 were studied. 6. 6. The data obtained in these studies are evaluated in terms of the cell architecture, selective permeability of the mitochondrial membranes and the action of adenylate kinases. From these evaluations a tentative mechanism is suggested to explain the Crabtree Effect.
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