Abstract
The aim of this work was to study the structure of the MyoD1 gene in sheep Stavropol breed.to identify polymorphisms associated with meat productivity. We has investigated 15 rams (n = 15) at the age of one year. Sequencing was performed using a genomic sequencer GS Junior (Roche, USA). Sequencing was performed using the genome sequencer GS Junior (Roche, USA). The fragments that were obtained by sequencing, mapped to Ovis aries reference genome assembly Ovis Aries oviAri3 (The National Center for Biotechnology Information. Genome. (2012) Ovis Aries (sheep), 2015) by software GS Reference Mapper v2.9 (Roche, USA). In the study, the regulatory regions were identified twenty-one single nucleotide substitution (SNP), of whichsix SNP discovered for the first time. Most often in the examined animals there are replacement c.-1687T>C; c.-1608C>T; c.-1603G>T; с*486A>C. To assess the impact of the structure of the gene MyoD1 on the meat productivity of sheep were selected by the following mutations: c.-1687T>C; c*2171A>G. With the aim of increasing the meat productivity of sheep of Stavropol breed, a markers SNP c.-1687T>C*2171A>G can be recommended for use in marker-oriented breeding.
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