Abstract
It is usually believed that apoptosis (Ap) and autophagy (Au) increases cell death during myocardial ischemia–reperfusion. In these experiments we have tested this assumption. Primary cultures of neonatal rat cardiomyocytes in our experiments contain 90.0±2.8% living (L), 3.3±1.09 necrotic (N), and 5.5±2.27 apoptotic (Ap) cells. After 30 min of anoxia (A) and 60 min reoxygenation (R) we have: L — 78.5±5.31%, N — 7.8±3.06% and Ap — 12.8±5.13 (P<0.05). After inhibition of apoptotic pathways by caspase-3 inhibitor N-acetyl-Asp-Glu-Val-Asp-al (DEVD, 100 μM) we observed that live cell population was not increased, but contrarily was decreased after A-R.
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