Interprétation des immunodots, myosite et sclérodermie systémique

Abstract

AbstractThe identification of autoantibodies associated with autoimmune diseases is a growing challenge in terms of diagnosis or prognosis. Indeed, these antibodies are part of the diagnostic criteria and score and some are associated with particular clinical forms impacting the prognosis.Given the number of autoantibodies that can be associated with autoimmune disease, multiparametric techniques to identify a panel of autoantibodies have been developed. Among these techniques, the immunodot has partially met this demand, but several studies have pointed to its lack of specificity in the diagnosis of myositis and systemic sclerosis.Two retrospective studies carried out at the University Hospital of Toulouse on autoantibodies in myositis and scleroderma perfectly illustrate the lack of specificity of these techniques and offer solutions to remedy this, including confrontation with the appearance in indirect immunofluorescence or by establishing new positivity thresholds.In the end, by their simplicity of implementation and their cost, immunodot techniques occupy a place of choice in the identification of autoantibodies. Their biological interpretation must however take into account their lack of specificity.