Abstract
The measurement of the efficiency of resonance energy transfer between two luminophores attached to the same macromolecular substrate is a promising tool for determining intramolecular distances if reasonable bounds can be placed upon the orientation factor k 2, To date, the analysis of such experiments has been based upon assumed average values of k 2. A critical review of this procedure is offered along with a method for estimating upper and lower limits for k 2 which derive from the freedom of motion of the luminophores as determined by polarized emission spectroscopy.
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