Abstract
Recruitment of Polycomb Group (PcG) proteins to DNA is vital for normal development. PcG proteins regulate expression of homeotic genes that are essential for axial body patterning during development. Earlier work showed that YAF2 is responsible for PcG recruitment to DNA, which is mediated by YY1 DNA binding. Our goal is to understand the role of CtBP, YAF2 and YY1 in context of recruitment of Polycomb complexes to DNA. Using a co‐immunoprecipitation (CoIP) approach, different protein‐protein interactions are studied using protein‐specific antibodies. This approach will allow us to recognize binding interactions between specific proteins. Moreover, our preliminary results show that CtBP might interact directly with the C‐terminal end of YY1 to recruit PcG proteins. CtBP represses transcription in a histone deacetylase‐dependent or ‐independent manner, depending on the promoter context. Based on this information, we are investigating the modulation of transcriptional activity of YY1 upon interaction with CtBP using luciferase‐based assays. In conclusion, our studies will provide information regarding the interplay between CtBP, YAF2, and YY1 and their overall relationship in recruitment of PcG proteins to DNA.Support or Funding InformationStart up funds from Penn State Brandywine
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