Interplay between the Brassica napus phytoglobin (BnPgb1), folic acid, and antioxidant responses enhances plant tolerance to waterlogging

Abstract

Oxygen deprivation by waterlogging reduces the productivity of several crop species, including the oil-producing crop Brassica napus L., which is highly sensitive to excess moisture. Among factors induced by oxygen deficiency are phytoglobins (Pgbs), heme-containing proteins known to ameliorate the response of plants to the stress. This study examined the early responses to waterlogging in B. napus plants over-expressing or down-regulating the class 1 (BnPgb1) and class 2 (BnPgb2) Pgbs. The depression of gas exchange parameters and plant biomass was exacerbated by the suppression of BnPgb1, while suppression of BnPgb2 did not evoke any changes. This suggests that natural occurring levels of BnPgb1 (but not BnPg2) are required for the response of the plants to waterlogging. Typical waterlogging symptoms, including the accumulation of reactive oxygen species (ROS) and the deterioration of the root apical meristem (RAM) were attenuated by over-expression of BnPgb1. These effects were associated with the activation of antioxidant system and the transcriptional induction of folic acid (FA). Pharmacological treatments revealed that high levels of FA were sufficient to revert the inhibitory effect of waterlogging, suggesting that the interplay between BnPgb1, antioxidant responses and FA might contribute to plant tolerance to waterlogging stress.