Interplay between proteases and protease inhibitors in the sea fan—Aspergillus pathosystem

Abstract

Environmental conditions greatly impact the dynamics of host–pathogen relationships, affecting outbreaks and emergence of new diseases. In corals, epizootics have been a main contributor to the decline of reef ecosystems and rising sea surface temperatures associated with climate change are thought to exacerbate virulence of pathogens and/or compromise the immune responses of coral hosts. Among pathogens strategies, protease secretion is a common virulence factor that promotes colonization of the host. As such, protease inhibitors play an invaluable role to the host for protection against pathogen invasion. This study describes changes in activity of proteases secreted by the fungal pathogen, Aspergillus sydowii, and protease inhibitors of Gorgonia ventalina under ambient and elevated temperatures and health conditions (disease and healthy). G. ventalina colonies were collected from Florida Keys, Florida (24° 34.138N, 81° 22.905W) or La Parguera, Puerto Rico (17° 56.091N, 67° 02.577W) in 2007 and 2012, respectively. At elevated temperatures, protease activity was significantly higher than at ambient temperatures in A. sydowii. Temperature stress did not induce a change in protease inhibitor activity, but in healthy G. ventalina colonies, inhibitor activity against proteases was higher than in diseased individuals. Healthy colonies appear capable of resisting proteolytic activity, but diseased individuals are still likely affected by pathogen infections. These data suggest that rising sea surface temperatures due to climate change may increase levels of virulence (protease activity) of A. sydowii, while immunity (protease inhibitor) of G. ventalina may not be affected.