Abstract
Histone H1 is an evolutionarily conserved linker histone protein that functions in arranging and stabilizing chromatin structure and is frequently fused to a fluorescent protein to track nuclei in live cells. In time-lapse analyses, we observed stochastic exchange of photoactivated Dendra2-histone H1 protein between nuclei within the same cellular compartment. We also observed exchange of histones between genetically distinct nuclei in a heterokaryon derived from fusion of strains carrying histone H1-RFP or H1-GFP. Subsequent analysis of the resulting uninucleate conidia containing both RFP- and GFP-labeled histone H1 proteins showed only parental genotypes, ruling out genetic recombination and diploidization. These data together suggest that the linker histone H1 protein can diffuse between non-daughter nuclei in the filamentous fungus Aspergillus nidulans.
Highlights
Histones are members of an ancient family of proteins whose roles have been constantly redefined since their discovery
When a dormant asexual spore of A. nidulans is furnished with a carbon source, it breaks dormancy, begins to swell, and a germ tube emerges
Throughout extension and partitioning, mitosis continues in the tip cell, while mitosis is arrested in subapical compartments until the compartment branches [13,14]
Summary
Histones are members of an ancient family of proteins whose roles have been constantly redefined since their discovery. These data from photoactivation experiments suggest that histone H1 proteins can localize to daughter and non-daughter nuclei within a hyphal compartment following mitosis.
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