Abstract
Myriocin, isolated from Bacillus amyloliquefaciens LZN01, can inhibit the growth of Fusarium oxysporum f. sp. niveum (Fon) by inducing membrane damage and targeting intracellular molecules. In the present study, the mechanism of myriocin entry into Fon cells was investigated. Fluorescence images demonstrated that internalization of myriocin was decreased by low temperature, NaN3 and Brefeldin A. The mechanism of myriocin entry into Fon cells was revealed by analyses of transcriptome and proteome. A total of 422 common differentially expressed genes (DEGs) were identified. Most of DEGs were assigned to transport and catabolism, folding, sorting and degradation, and enrichment analysis of some DEGs was closely related to transmembrane transport and vesicle transport. The combined analysis between the transcriptome and proteome showed that the expression levels of some related genes and proteins, mainly those related to oxidative phosphorylation, ABC transporters, micro chromosome maintenance protein, adaptor proteins etc., were affected. The DEGs were further verified by reverse transcription quantitative polymerase chain reaction (RT-qPCR). In conclusion, myriocin’s entry into Fon cells depended partially on energy and involved endocytosis, and the expression of certain genes and proteins was affected.
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