Abstract
We have been working towards a 3 dimensional structural understanding of the Nuclear Pore Complex (NPC) with a view to investigating structural alterations associated with the molecular mechanism of transport across the nuclear envelope. FEISEM allows direct visualisation of changes in individual NPCs which will complement information from TEM 3D reconstructions. FEISEM has produced significant new information on the more peripheral elements of the NPC, most notably the nuclear pore basket or ‘fishtrap’ and the nuclear envelope lattice. NPC baskets have been recognised in both avian and insect species as well as amphibia and are likely to be evolutionarily conserved. In the salivary gland nuclei ofChironomusdifferent configurations of basket structure have been recognised undergoing interaction with Balbiani Ring particles during mRNA export. (Fig 1).The ability to observe and directly compare large numbers of NPCs on isolated NEs from either the cytoplasmic or nucleoplasmicface, at equivalent biological resolution to TEM has enabled us to access internal NPC organisation by surface imaging.
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