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Abstract
The acceptor specificity in the intermolecular transglycosylation of cyclodextrin glycosyltransferase (CGT-ase) was investigated using 18 kinds of monosaccharides and their derivatives. It is concluded on the basis of the experimental results that the requirement for an acceptor of the intermolecular transfer reaction catalyzed by CGT-ase is the pyranose structure having the same configurations of the C2-, C3- and C4-hydroxyl group as D-glucopyranose. Furthermore, it was showed that CGT-ase transfered the glycosyl residues only to C3-hydroxyl group of L-sorbose as acceptor and C4-hydroxyl group of D-glucose, D-xylose and 2-deoxy-D-glucose as acceptor. On the other hand, in the case of D-galactose as acceptor which is different from D-glucopyranose in the configuration of the hydroxyl group at C4 position, CGT-ase transfered the glycosyl residues to C1-, C3- and C2- (C4-) hydroxyl group of D-galactose in the proportion of 26 : 10 : 1.
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