Abstract
Positive-strand RNA viruses replicate their genomes in membrane-bound replication compartments. Brome mosaic virus (BMV) replicates in vesicular invaginations of the endoplasmic reticulum membrane. BMV has served as a productive model system to study processes like virus-host interactions, RNA replication and recombination. Here we present multiple lines of evidence showing that the structure of the viral RNA replication compartments plays a fundamental role and that recruitment of parental RNAs to a common replication compartment is a limiting step in intermolecular RNA recombination. We show that a previously defined requirement for an RNA recruitment element on both parental RNAs is not to function as a preferred crossover site, but in order for individual RNAs to be recruited into the replication compartments. Moreover, modulating the form of the replication compartments from spherular vesicles (spherules) to more expansive membrane layers increased intermolecular RNA recombination frequency by 200- to 1000-fold. We propose that intermolecular RNA recombination requires parental RNAs to be recruited into replication compartments as monomers, and that recruitment of multiple RNAs into a contiguous space is much more common for layers than for spherules. These results could explain differences in recombination frequencies between viruses that replicate in association with smaller spherules versus larger double-membrane vesicles and convoluted membranes.
Highlights
Viruses are intracellular parasites that depend on host cells for replication [1,2,3]
Prior work showed that intermolecular RNA recombination of Brome mosaic virus (BMV) RNA3 requires the recruitment element, including a bulged stem loop known as the box B, in both parental RNAs [10]
To distinguish between these possibilities, we introduced single nucleotide substitutions in one of the parental RNAs (B3∆30 pm) and mapped the distribution of crossover sites in the progeny (Figure 1)
Summary
Viruses are intracellular parasites that depend on host cells for replication [1,2,3]. Viral and host components affecting abundance andmutations features of affecting viral RNApolymerase influence both the silencing viral RNA sequences and properties frequency of RNA recombination and features of the progeny. Less is known about the role components of the structure the biggest impact are proteins involved in host binding, or viral replication compartments in viral RNA recombination. Positive-strand RNA viruses of plants and animals replicate their genomic RNA on remodeled properties influence the distribution of crossover sites [11,19]. Less is known about the role of the intracellular membranes For many such viruses, this genome replication occurs in virus-induced, structure of the viral replication compartments in viral RNA recombination. RNA1 and RNA2 encode the non-structural replication proteins 1a and 2apol, of endoplasmic reticulum membranes to protein form spherules, recruits viral RNA and the the 2apol respectively. Distribution of crossoversites sitesafter after one one yeast in in brome mosaic virusvirus (BMV)(BMV)
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
