Intermolecular interactions of influenza M1 proteins on the model lipid membrane surface: A study using the inner field compensation method

Abstract

M1 protein binding to the lipid bilayer membrane (BLM) was recorded by the inner field compensation technique as a change of the boundary potential. After the protein was added to the bulk solution, the M1 adsorption produced a slow increase in boundary potential to a stationary value that was reached within the time period dependent on the quantity of the added protein. The stationary value of the potential grew with the decrease of pH or KCl concentration in the medium and was higher in the presence of negatively charged lipids in the BLM. It was shown that the potential growth with the decrease of pH is due to an increase of M1 molecule charge and not due to the increase of the M1 surface concentration or to the change of lipid charge. As the potential did not change after the removal of the protein from the bulk solution, we consider the protein adsorption on the BLM irreversible. The obtained results suggest that the protein adsorption is influenced by both electrostatic and hydrophobic interactions of M1 molecules with each other and with lipid membrane. We offer a mechanism of dissociation of the viral shell formed by M1 matrix protein. The protein shell is destabilized due to electrostatic repulsion of protein molecules caused by the increase of their positive charge.