Intermittent warming of peaches reduces chilling injury by enhancing ethylene production and enzymes mediated by ethylene

Abstract

SUMMARYPeaches (Prunus persica ‘Hermoza’) were ripened at 208>C after harvest and either stored at 08C for four weeks (control) to induce chilling injury or given intermittent warming (IW) on the twelfth day of storage (28C for 24.h) to alleviate chilling injury (CI). Continuously stored fruit from control developed wo olliness, a CI disorder, during ripening at 208C after cold storage while only a small percentage of IW fruit developed woolliness. CI fruit produced less ethylene during ripening after storage, and this inhibited ethylene production was closely tied with woolliness development. The IW treatment caused enhanced ethylene production in the fruit when returned to 08C and the ethylene remained higher than control fruit until the end of the storage period. IW also induced the messages for 1-aminocyclopropane-1-carboxylic acid oxidase (ACO) and 1-aminocyclopropane-1-carboxylic acid synthase (ACS) in the ethylene synthesis pathway. IW also elevated the mRNA of the cell wall degrading enzymes polygalacturonase (PG) and endo-1,4-gluconase (EGase). The mRNAs of the cell wall enzymes and the enzymes in the ethylene synthesis pathway remained higher even after 5.d at 08 C following the IW treatment. It is suggested that IW maintained the fruit tissue capacity to ripen normally by preventing inhibition of the ethylene synthesis pathway which occurred in the control fruit after extended storage.