Abstract
A method involving the gas chromatographic separation of the trimethylsilyl derivatives of possible intermediates in the biosynthesis of cyanogenic glucosides and the determination of the radioactivity in the individual compounds by use of a gas proportional counter is described. The trimethylsilyl derivatives were prepared by reacting lyophilized assay mixtures with bis-(trimethylsilyl)-trifluoroacetamide using acetonitrile as a reaction solvent. Gas chromatographic separation was accomplished with a 3% SP-2250 glass column and temperature programming. The organic material in the chromatographic effluent was oxidized to carbon dioxide and water by passing it through an oven containing cupric oxide, and the carbon dioxide was counted in a gas proportional counter. Structures of the trimethylsilyl derivatives were confirmed by combined gas chromatography—mass spectrometry.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
