Abstract
Using the new method of sucrose density gradient centrifugation technique for the analysis of intact cell DNA without artificial scission, the discontinuous replication of DNA in cultured mouse mammary carcinoma cells was investigated. The results indicated the sequence of elongation of short fragments in the process of replication and several intermediate fragments were found. Their sizes were 20S, 40S, 70–100S, 280S and the bulk of the cell DNA was 380S.
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