Abstract
INTERMEDIATE COMPOUND FORMATION WITH PEROXIDASE AND STRONG OXIDIZING AGENTS
Highlights
The intermediate compounds were quantitatively reduced back to the ferriperoxidase by ferrocyanide ions in the case of HzOz, MeOOH, HOCl, HOBr, and NaCIOz; examples are shown in Figs. 1 and 2 for the HRP-HOC1 system and the CcP-NaC102 system respectively
Under the experimental conditions employed these compounds are indistinguishable from the mixture of Compounds I and II in the case of horseradish peroxidase and Compound II in the case of cytochrome c peroxidase which are formed when the enzymes react with HzOz or CH300H
The ability of five of the above oxidizing agents to replace Hz02 as the oxidizing agent in the system guaiacol-HgOz-horseradish peroxidase has been investigated by measuring the rate of tetraguaiacol production spectrophotometrically
Summary
Since N&102, ClOz, and KBr03 possess 3; 4, and 5 oxidizing equivalents compared to the 2 of hydrogen peroxide, a factor accounting for t,his should be introduced if complete reduction occurred 6.7 rnM guaiacol and 2.6 X 10mg M HRP at pH 7.0
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