Abstract
Lipidic phases, containing ‘lipidic particles’ (dioleoylphosphatidylethanolamine/cholesterol/dioleoylphosphatidylcholine and cardiolipin/dimyristoylphosphatidylcholine in the presence of Ca 2+) have been investigated by preparing thin films from a suspension of sonicated vesicles. These thin films were vitrified and observed ‘directly’ by cryo-electron microscopy in their hydrated form. The thin films show various fusion products and fusion intermediates such as lipidic particles.
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