Abstract
We recently reported that interleukin-4 (IL-4) inhibited prostanoid synthesis through inhibiting cyclooxygenase 2 biosynthesis. In the present study, we examined the effect of IL-4 on the expression of cytosolic phospholipase A2(cPLA2). The amounts of protein and mRNA of cPLA2were determined by western blotting and reverse transcription polymerase chain reaction (RT-PCR), respectively. Although interleukin-1α (IL-1α) and tumor necrosis factor α (TNFα) had little effect on the biosynthesis of cPLA2in phorbol myristate acetate (PMA)-differentiated U937 cells, lipopolysaccharide (LPS) increased the protein level of cPLA2in a dose-dependent manner. IL-4 inhibited the increased synthesis of cPLA2at the mRNA level. In addition, IL-4 inhibited the biosynthesis of cPLA2in untreated or LPS treated freshly prepared rheumatoid synovial cells at the mRNA level. These findings suggest that IL-4 inhibits prostanoid synthesis through inhibiting the expression of both cPLA2and cyclooxygenase 2.
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