Interleukin-27 induces a STAT1/3- and NF-κB-dependent proinflammatory cytokine profile in human monocytes.

Abstract

Interleukin-27 Induces a STAT1/3- and NF-κB-dependent Proinflammatory Cytokine Profile in Human MonocytesJournal of Biological ChemistryVol. 285Issue 32PreviewIL-27 is a heterodimeric cytokine bridging innate and adaptive immunity by playing a role in the activation of naive T cells and in development of Th1 cells. Additionally, recent evidence supports a role for IL-27 in the activation of monocytic cells. Both pro-inflammatory and anti-inflammatory activities have been attributed to IL-27; however, the role played by IL-27 in the activation of human monocytic cells in terms of cytokine production has not been well described. Our results show that IL-27 is a strong inducer of proinflammatory cytokine and chemokine expression, including enhancement of IL-6, IP-10, MIP-1α, MIP-1β, and TNF-α expression in human primary monocytes. Full-Text PDF Open Access VOLUME 285 (2010) PAGES 24404–24411 PAGE 24408: The flow cytometry panel in Fig. 2B is a duplicate of that shown in Fig. 3B. The data in B of both figures show the same trend but from two different cell types. Specifically, the data in B for both Figs. 2 and 3 is confirmatory data showing that IL-27 induces IL-6, IP-10, MIP-1α, MIP-1β, and TNF-α expression in primary monocytes (Fig. 2) and THP-1 cells (Fig. 3). Inadvertently, the data for the THP-1 cells (Fig. 3B) were duplicated in the monocyte figure (Fig. 2B), and this was missed by the reviewers and our inspection of the paper prior to publication. We are very concerned by this duplication and submit an erratum for Fig. 2, with the correct flow cytometry panel (B), showing the IL-27-induced cytokine expression in primary monocytes. No changes are necessary to the manuscript text as there is no change to the meaning or interpretation of the data.