Abstract
Background: Tumor cell adhesion to extracellular matrix (ECM) proteins plays an important role in tumor invasion and metastasis. We studied integrin expression in human pancreatic cancer cell lines and its alteration by Interleukin-1α (IL-1α) to determine the adhesion mechaninsm of metastatic human pancreatic cancer cells to ECM proteins.Methods: The expression of integrin subunits and their alteration by IL-1α were studied by flow-cytometric analysis and cellular-ELISA in metastatic human pancreatic cancer cell lines. Cancer cell adhesion to ECM proteins was assayed to determine if increased integrin expression actually affected adhesive interaction between cancer cells and putative integrin ECM ligands.Result: The α6β1subunit expressed in metastatic cancer cells was enhanced by IL-1α. Metastatic cancer cells also showed preferential adherence to laminin compared to nonmetastatic cancer cells, and this was enhanced by IL-1α.Conclusions: In pancreatic cancer, the enhancement of α6β1-integrin by IL-1α through IL-1RI plays an important role in metastasis formation.
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