Interleukin-6 gene–174g/C polymorphism (rs1800795) in pediatric septic shock: a prospective observational study

Abstract

Objective The present study was conducted to evaluate interleukin-16 (IL-6)–174 G/C polymorphism (rs1800795) as a predictor of outcomes of pediatric septic shock. Patients and methods This prospective observational study included 101 children having severe sepsis or septic shock admitted to the Pediatric Intensive Care Unit of a teaching university hospital starting from September 1, 2019 to March 31, 2020. A blood sample of 2 ml of whole blood was collected in EDTA tube on day 1 of admission. Genomic DNA was extracted from peripheral-blood mononuclear cells. IL-6–174G/C single-nucleotide polymorphism (rs1800795) genotyping was carried out using real-time quantitative-PCR by 5’-nuclease allelic-discrimination assay. Results The observed genotype frequencies are in equilibrium with Hardy–Weinberg equation (P=0.525). Regarding IL-6 genotypes, CC, CG, and GG represents 5, 39.6, and 55.4%, respectively. There was no significant difference between survived and deceased group as regards the polymorphisms CC, CG, or GG. There was no significant difference between both groups as regards C and G allele. There was no significant difference between CC, CG, and GG polymorphisms as regards mechanical-ventilation days, duration of Pediatric Intensive Care Unit stay, Pediatric Index of Mortality 2 score, C-reactive protein, white blood cells, as well as cultures. Conclusion IL-6–174G/C polymorphism (rs1800795) cannot predict mortality and did not affect outcomes in pediatric septic shock.