Interleukin 4 (IL4), IL10 and IL13 Inhibit in vitro Cytokine Secretion by Acute Myelogenous Leukemia Blasts in the Presence of Exogenous Hematopoietic Growth Factors

Abstract

The cytokines Interleukin 4 (IL4), IL10 and IL13 have divergent effects on acute myelogenous leukemia (AML) blast proliferation in vitro, and their final effect (enhancement/inhibition/no effect) depends on individual differences between patients and the presence of other exogenous cytokines. In contrast to these divergent effects on blast proliferation, all three cytokines inhibit constitutive (spontaneous) AML blast cytokine secretion independent of their effects on spontaneous blast proliferation. In the present study we investigated whether the inhibitory effects on AML blast cytokine secretion could be reversed by exogenous granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF) or IL3. Our results demonstrated that IL4/IL10/IL13 inhibited AML blast secretion of IL1β, IL6 and Tumor necrosis factor α (TNFα) even in the presence of exogenous G-CSF, GM-CSF and IL3. We conclude that although exogenous G-CSF, GM-CSF and IL3 can modulate the effects of IL4/IL10/IL13 on AML blast proliferation, the IL4/IL10/IL13-induced inhibition of AML blast cytokine secretion is not modulated/reversed by the presence of these exogenous hematopoietic growth factors.