Interleukin-33 promotes proliferation of primary human lung fibroblasts

Abstract

Event Abstract Back to Event Interleukin-33 promotes proliferation of primary human lung fibroblasts Alexander Bocharov1* and Olga Kornilova1 1 National University of Pharmacy, Ukraine Interleukin (IL)-33 is a multifunctional cytokine that belongs to the IL-1 cytokine family and expressed by multiple organs and cell types in humans and mice. Recent studies have showed that IL-33 plays an etiological role in several fibrotic disorders and may be involved in the pathogenesis of chronic respiratory diseases, such as asthma, and pulmonary fibrosis. It has been reported that IL-33–induced cutaneous fibrosis is associated with increased fibroblast proliferation and altered expression of extracellular matrix-modifying genes. However, the role of IL-33 in regulating of functions of lung fibroblasts remains unclear. In the present study, we examined the effect of recombinant human interleukin (IL)-33 on proliferation of human lung fibroblasts. Five primary lines of normal adult human lung fibroblasts were cultured for 3-7 days in the presence of increasing concentrations of IL-33 (1, 2, 10, 50, 100 and 300 ng/ml). The proliferation of human lung fibroblasts was analyzed using non-radioactive cell proliferation assay (MTT). We have observed that normal human lung fibroblasts responded, in a dose-dependent manner, to treatment with recombinant human IL-33, by increasing proliferation rates 1.5- to 2.3 fold compared with non-stimulated control. The maximum effect of IL-33 on fibroblast proliferation was observed in cytokine concentrations range from 2 ng/ml to 100 ng/ml. These results suggest that IL-33 may play an important role in the regulation of human lung fibroblast proliferation. Human lung fibroblasts activated by IL-33 may act as effector cells not only in the pathogenesis of lung diseases but also in lung remodeling processes. Keywords: interleukin -33, human lung fibroblasts, proliferation, Pulmonary Fibrosis, fibrotic disorders Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Innate immunity Citation: Bocharov A and Kornilova O (2013). Interleukin-33 promotes proliferation of primary human lung fibroblasts. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00565 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 02 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Alexander Bocharov, National University of Pharmacy, Kharkov, Ukraine, a.a.botcharov@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Alexander Bocharov Olga Kornilova Google Alexander Bocharov Olga Kornilova Google Scholar Alexander Bocharov Olga Kornilova PubMed Alexander Bocharov Olga Kornilova Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.