Interleukin-27 levels and its Polymorphism in Hepatocellular carcinoma associated with Hepatitis C

Nahla Anber,Maysaa El Sayed Zaki,Mona El Wassefy

doi:10.21608/nrmj.2020.73434

Nahla Anber, Maysaa El Sayed Zaki + Show 1 more

Open Access

https://doi.org/10.21608/nrmj.2020.73434

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Abstract

The prevalence of Hepatocellular carcinoma (HCC) in Egypt represents about 11% of the gastrointestinal tract malignancies. The development of HCC usually is preceded by chronic infections with Hepatitis C virus (HCV). Interleukin-27 (IL27) is implicated in the antiviral immunity. Little is known about the use of IL27 as a biomarker for the development of HCC associated with HCV. Moreover, the role of IL-27 rs153109 gene polymorphism in the development of HCC associated with HCV has not been well studied yet. A case control study included three groups; Group I, 60 healthy control patients, Group II, 60 patients with cirrhosis associated with HCV infection, and Group III, included 60 patients with HCC associated with HCV. ELISA technique was used to measure serum IL27, while restriction fragment length polymorphism (RFLP) was used to detect single-nucleotide polymorphisms (SNP). Results showed that the serum levels of IL27 is significantly higher in both cirrhotic and HCC groups. G allele is significantly associated with HCC and cirrhotic cases, compared with the healthy control group. Current results encourage the use of IL 27 as a diagnostic marker for both cirrhotic and HCC diseases. The aims of current work were to study the serum levels of IL27 in HCV positive hepatic patients, to evaluate its role as a biomarker for early detection of HCC, and to find any association of rs153109 with the hepatic diseases.

Highlights

Hepatocellular carcinoma (HCC) is defined as a common malignancy that spreads worldwide
The objectives of the present study were; to compare the serum levels of IL27 in patients with cirrhosis and HCC associated with hepatitis C, to evaluate IL27 role as a biomarker for early detection of HCC, and to study the genetic polymorphism of IL27 rs153109 associated with cirrhosis and HCC compared to the healthy control
The present study comprised of 60 patients with liver cirrhosis (LC) and 60 HCC cases, in addition to 60 healthy control participants of matched age and gender

Summary

Introduction

Hepatocellular carcinoma (HCC) is defined as a common malignancy that spreads worldwide. Dai et al, (2014) reported that it represents the sixth most prevalent cancer and the third common cause of deaths due to malignancy. In Egypt, the prevalence of HCC is around 11 % of the gastrointestinal tract malignancies, and represents about 2 % of all malignancies (Mokhtar et al, 2007) The development of HCC is usually proceeded by chronic infections with hepatitis C HCV) or/and hepatitis B viruses (HBV) (Gomaa et al, 2014). Cytokines production is subjected to genetic variations among the affected individuals. Variations in a single nucleotide due to single-nucleotide polymorphisms (SNPs) in the cytokine genes affect cytokine productions/ functions, leading to the disturbance of the immune response to the viruses infections, and may contribute to HCC development (Dondeti et al, 2016)

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