Abstract
Dysfunctions in adipose tissue cells are responsible for several obesity-related metabolic diseases. Understanding the process of adipocyte formation is thus fundamental for understanding these diseases. The adipocyte differentiation of adipose-derived stem/stromal cells (ADSCs) showed a reduction in the mRNA level of the interleukin 21 receptor (IL21R) during this process. Although the receptor has been associated with metabolic diseases, few studies have examined its function in stem cells. In this study, we used confocal immunofluorescence assays to determine that IL21R colocalizes with mitochondrial protein ATP5B, ALDH4A1, and the nucleus of human ADSCs. We demonstrated that silencing and overexpression of IL21R did not affect the cell proliferation and mitochondrial activity of ADSCs. However, IL21R silencing did reduce ADSC adipogenic capacity. Further studies are needed to understand the mechanism involved between IL21R and the adipogenic differentiation process.
Highlights
Adipose-derived stem/stromal cells (ADSCs) are considered ideal for application in regenerative therapies for several reasons, including the ease and efficiency with which they can be extracted, handled, and expanded [1]
We previously demonstrated that the amount of interleukin 21 receptor (IL21R) mRNA associated with polysomes decreases during early adipogenic differentiation [5]
The presence of IL21R mRNA was previously demonstrated in human ADSCs [5, 10], the protein expression and intracellular localization of IL21 receptors remain unknown
Summary
Adipose-derived stem/stromal cells (ADSCs) are considered ideal for application in regenerative therapies for several reasons, including the ease and efficiency with which they can be extracted, handled, and expanded [1]. They are a study model for understanding the mechanisms of cell differentiation. Adipocytes are the mature cells that make up most of the adipose tissue and are formed by adipogenesis, a complex and fine-tuned process (reviewed by [3]). We previously demonstrated that the amount of interleukin 21 receptor (IL21R) mRNA associated with polysomes decreases during early adipogenic differentiation (logFC = −1:723, FDR = 8:22E − 04) [5]. Interleukin 21 interacts with IL21R (its receptor) and transduces the signal into cells through proteins JAK1, Stem Cells International
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