Abstract
Proliferating cell nuclear antigen (PCNA) is an auxiliary protein required for leading strand DNA synthesis by polymerase delta. Steady-state levels of PCNA RNA increase during interleukin 2 stimulated G1 activation of cloned T cells (L2 cells) and correlate with expression of the PCNA protein. We demonstrate that the initial (G1) rapid rise in PCNA RNA levels is predominantly related to increased transcription. The slower rate of increase in PCNA RNA at the initial G1-S transition is related to transcription as well as increased stability of the PCNA message and partially requires protein synthesis. These data provide a foundation for further investigation of the mechanisms of increased stability and transcription of PCNA.
Highlights
Proliferating cell nuclear antigen (PCNA) is an auxiliary protein required for leading strand DNA synthesis by polymerase delta
Changes in PCNA RNA during Cell Growth-We previously demonstrated that PCNA-associated immunofluorescence is tightly associated with proliferation, not merely S-phase, of L2 cells stimulated with IL2 [6]
PCNA RNA levels increase at the Go-G1 entrance to the cell cycle [9,10,11] and, as we have shown in cloned T lymphocytes (L2 cells) stimulated with IL2 to progress through the cell cycle from G1
Summary
Proliferating cell nuclear antigen (PCNA) is an auxiliary protein required for leading strand DNA synthesis by polymerase delta. S transition is related to transcription as well as increased stability of the PCNA message and partially requires protein synthesis. Proliferating cell nuclear antigen (PCNA),’ identified as the auxiliary protein for DNA polymerase 6 (1, Z), enhances the processivity of polymerase 6 during SV40 replication in vitro [3] and of calf thymus DNA polymerase with synthetic primed poly d(A)/poly d(T) templates [2]. PCNA RNA levels increase during cell cycle progression of IL2-stimulated cloned murine T lymphocytes [6], phytohemagglutinin-stimulated human peripheral blood lymphocytes [9], mouse 3T3 cells stimulated with serum [10] or platelet-.
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