Abstract
Article history: Received 31 July 2015 Received in revised form 24 September 2015 Accepted 28 September 2015 Available online 03 October 2015 atria, RV free wall was removed from left ventricle (LV). Each ventricle was weighed and the ratio of RV weight to LV plus septum weight (RV/LV + Sp) was calculated. Lungs were fixed with 4% buffered paraformaldehyde for 24 h, embedded in paraffin, and cut into 4-μm-thick sections. Hematoxylineosin (HE) and Elastica van Gieson (EVG) stainings were performed using standard protocols. Immunofluorescence staining was performed using a primary anti-α-smooth muscle actin (α-SMA) mouse antibody
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