Interleukin-17A and interleukin-17F mRNA expression in peripheral blood mononuclear cells of patients with multiple sclerosis.

Abstract

Multiple sclerosis (MS) is a CD4+ T cell-mediated autoimmune disease affecting the central nervous system (CNS). It was previously believed that Th1 cells were pathogenic T cells in experimental autoimmune encephalomyelitis (EAE). However, the functional role of Th1 cells in EAE has been reconsidered upon the discovery of IL-17-producing T cells which are considered as dominant effectors for inducing autoimmune tissue inflammation. The objective of this study was to assess the role of IL-17A and IL-17F in MS pathogenesis. We evaluated mRNA expression of IL-17A and IL-17F in thirty-five Iranian patients with relapsing-remitting MS (RRMS) and twenty-five healthy controls by Quantitative Real Time PCR. The results of this study showed a twenty-fold increase in the expression of IL-17A mRNA in MS patients compared to the control group (p < 0.0001). IL-17F mRNA expression in MS patients was thirty three-times greater than the control group (p = 0.0008). IL-17A mRNA expression in the periphery was positively correlated with the expression of IL-17F transcripts in MS patients and controls (p < 0.01and p < 0.05, respectively). These results indicate the critical role of Th17- mediated cytokines in the development of MS which was classically considered as a Th1-mediated disorder. The results of this study showed, for the first time, the importance of IL-17F in MS immunopathogenesis.