Abstract
Objective: To study the relationship between Interleukin-17 receptor C (IL-17RC) gene polymorphism and ischemic stroke (IS).Methods: Three hundred cases of IS patients and 300 cases of the healthy controls were selected. Serum of IS patients and the controls was collected. The relative mRNA levels of IL-17, IL-17RC, IL-6, IL-8, G-CSF and granulocyte-macrophage colony stimulating factor (GM-CSF) by qRT-PCR. The protein expression of IL-17 and IL-17RC was determined by Western blotting. IL-17RC genotype was identified by PCR amplification. The proportion of IL-17RC, SNP and re37511 in IS and control group was determined. The treatment effect on IS and prognosis of patients with IL-17RC, SNP and re37511 was compared.Results: The relative mRNA levels of IL-17, IL-17RC, IL-6, IL-8, G-CSF and GM-CSF in IS group were significantly higher than the control group. The protein expression of IL-17 and IL-17RC in IS group was also markedly higher than the control group. The proportion of IL-17RC re37511 in IS group was much larger than control group and proportion of IL-17RC much less. The percent of poor treatment effect in re37511 was much larger than IL-17RC. The percent of death and recrudescence in patients with IL-17RC re37511 was the highest.Conclusion: IS up-regulates the expression of IL-17 and IL-17RC. IL-17RC re37511 indicates the patients have a poorer treatment effect and prognosis.
Highlights
Stroke is the most common reason of permanent disability in adults in the world [1]
We aimed to explore the relationship between Interleukin-17 receptor C (IL-17RC) gene polymorphism and development of Ischemic stroke (IS)
To explore the relationship between inflammation and IS, we determined the mRNA levels of IL-17, IL-17RC, IL-6, IL-8, G-CSF and granulocyte-macrophage colony stimulating factor (GM-CSF) by Quantitative real time PCR (qRT-PCR)
Summary
Stroke is the most common reason of permanent disability in adults in the world [1]. After stroke for 3 months, 20% of the survivors need nurse and 15–30% become permanently disabled [2]. Pathogenesis of stroke includes a variety of mechanisms, but inflammation and ischemic damage are the major reasons. Ischemic stroke (IS) is a serious disease and usually associated with a high rate of mortality [3]. Atherosclerosis is one of the main causes of IS and its important pathophysiological basis is inflammation of blood vessels. The levels of pro-inflammatory genes can affect the occurrence and development of IS
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