Interleukin-12 as a biomarker of the beneficial effects of food restriction in mice receiving high fat diet or high carbohydrate diet.

C.B De Almeida-Souza,M.A.R.C.P Silva,C.R Schamber,R.B Bazotte,M.M Antunes,G Godoy

doi:10.1590/1414-431x20187900

C.B De Almeida-Souza, M.A.R.C.P Silva + Show 4 more

Open Access

https://doi.org/10.1590/1414-431x20187900

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Abstract

The impact of food restriction (FR) during 56 days on serum levels of cytokines in mice fed a high-fat diet (HFD) or high-carbohydrate diet (HCD) were evaluated. The amount of food was reduced 50% for HFD-FR and HCD-FR groups compared to mice receiving free access to HFD (HFD group) or HCD (HCD group). We quantified the serum levels of basic fibroblast growth factor, granulocyte-macrophage colony-stimulating factor, inducible protein 10, interferon γ, interleukin 1α (IL-1α), IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12, IL-13, IL-17, keratinocyte chemoattractant, macrophage inflammatory protein-1α, monocyte chemotactic protein 1, monokine induced by IFN-γ, and tumor necrosis factor α. Only IL-12 levels were lower (P<0.05), for both HFD-FR (HFD-FR vs HFD) and HCD-FR (HCD-FR vs HCD). Therefore, IL-12 levels could be considered a biological marker of the beneficial effects of FR.

Highlights

Excessive caloric consumption as a high-fat diet (HFD) or high-carbohydrate diet (HCD) is largely responsible for the epidemics of chronic diseases associated with inflammation
The impact of food restriction (FR) in mice receiving HFD or HCD during 56 days on serum levels of basic fibroblast growth factor (FGF-basic), granulocytemacrophage colony-stimulating factor (GM-CSF), inducible protein 10 (IP-10), interferon g (IFN-g), interleukin 1a (IL-1a), IL-1b, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12, IL-13, IL-17, keratinocyte chemoattractant (KC), macrophage inflammatory protein-1a (MIP-1-a), monocyte chemotactic protein 1 (MCP-1), monokine induced by IFN-g (MIG), and tumor necrosis factor a (TNF-a) were evaluated
We investigated the effects of food restriction on serum pro-inflammatory and antiinflammatory cytokines levels in mice

Summary

Introduction

Excessive caloric consumption as a high-fat diet (HFD) or high-carbohydrate diet (HCD) is largely responsible for the epidemics of chronic diseases associated with inflammation. The impact of FR in mice receiving HFD or HCD during 56 days on serum levels of basic fibroblast growth factor (FGF-basic), granulocytemacrophage colony-stimulating factor (GM-CSF), inducible protein 10 (IP-10), interferon g (IFN-g), interleukin 1a (IL-1a), IL-1b, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12, IL-13, IL-17, keratinocyte chemoattractant (KC), macrophage inflammatory protein-1a (MIP-1-a), monocyte chemotactic protein 1 (MCP-1), monokine induced by IFN-g (MIG), and tumor necrosis factor a (TNF-a) were evaluated. HCD and HFD groups had free access to food while the amount of food was reduced 50% for HCD-FR or HFD-FR for 56 days.

Results

Conclusion