Interleukin-10 gene polymorphisms (-1082 A/G, -819 C/T, -592 C/A) in primary Sj�gren's syndrome

Abstract

Event Abstract Back to Event Interleukin-10 gene polymorphisms (-1082 A/G, -819 C/T, -592 C/A) in primary Sjögren's syndrome Edith Oregon-Romero1*, Mirna Vázquez-Villamar1, Diana C. Salazar-Camarena1, Beatriz A. Treviño-Talavera1, Gerardo Orozco-Barocio2, José F. Muñoz-Valle1, Yeminia M. Valle-Delgadillo1 and Claudia A. Palafox-Sánchez1, 2 1 Universidad de Guadalajara, Mexico 2 Hospital General de Occidente, Servicio de Reumatología, Mexico Introduction: Primary Sjögren´s syndrome (pSS) is an autoimmune disease of unknown etiology affecting primarily salivary and lacrimal exocrine glands. It is characterized by a progressive lymphocytic infiltration where B cells produce a great variety of antibodies, including those against ribonucleoproteins Ro and La. IL-10 plays an important role in pSS by promoting the differentiation of B cells inducing antibody production. Three polymorphic sites located at positions -1082 (A/G), -819 (C/T) and -592 (C/A) in the IL-10 promoter show a linkage disequilibrium and form haplotypes that may influence the IL-10 production and consequently an increased activity of the disease. Objective: To determine the frequency of haplotypes in the IL-10 promoter gene in patients with pSS. Methodology: The study included 113 Mexican mestizo patients with pSS, classified according to the American-European Consensus criteria, from the Department of Rheumatology (Hospital General de Occidente, SSJ). As control group, 154 clinically healthy subjects were included. The polymorphisms were genotyped by PCR/RFLP technique. Statistical analysis was carried out with the Stata 9.0 and EmHapFre software. Results: Polymorphisms were in Hardy–Weinberg equilibrium (-1082 A/G p=0.8377, -819C/T p= 0.1174, -592 C/A p=0.7953) and showed high linkage disequilibrium (100%, pc=1.42 x 10-34).The most frequent haplotypes were: ACC (41-61%), ATT (21.38%), GTA (14.86%), and GCC (11.18%). GTC haplotype was associated with a 4.35-fold less probability to develop pSS [OR; 95%CI: 0.23 (0.064-0.792) p= 0.01]. Conclusion: Our results suggest that carriers of the GTC haplotype have a protective status for the development of pSS. Keywords: primary Sjögren´s syndrome, Antibodies, IL-10 promoter, Linkage Disequilibrium, Haplotypes Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Immune-mediated disease pathogenesis Citation: Oregon-Romero E, Vázquez-Villamar M, Salazar-Camarena DC, Treviño-Talavera BA, Orozco-Barocio G, Muñoz-Valle JF, Valle-Delgadillo YM and Palafox-Sánchez CA (2013). Interleukin-10 gene polymorphisms (-1082 A/G, -819 C/T, -592 C/A) in primary Sjögren's syndrome. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00346 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 15 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Edith Oregon-Romero, Universidad de Guadalajara, Guadalajara, Mexico, oregon_edith@hotmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Edith Oregon-Romero Mirna Vázquez-Villamar Diana C Salazar-Camarena Beatriz A Treviño-Talavera Gerardo Orozco-Barocio José F Muñoz-Valle Yeminia M Valle-Delgadillo Claudia A Palafox-Sánchez Google Edith Oregon-Romero Mirna Vázquez-Villamar Diana C Salazar-Camarena Beatriz A Treviño-Talavera Gerardo Orozco-Barocio José F Muñoz-Valle Yeminia M Valle-Delgadillo Claudia A Palafox-Sánchez Google Scholar Edith Oregon-Romero Mirna Vázquez-Villamar Diana C Salazar-Camarena Beatriz A Treviño-Talavera Gerardo Orozco-Barocio José F Muñoz-Valle Yeminia M Valle-Delgadillo Claudia A Palafox-Sánchez PubMed Edith Oregon-Romero Mirna Vázquez-Villamar Diana C Salazar-Camarena Beatriz A Treviño-Talavera Gerardo Orozco-Barocio José F Muñoz-Valle Yeminia M Valle-Delgadillo Claudia A Palafox-Sánchez Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.