Abstract
Cytokines play critical roles in the pathogenesis of Polycystic Ovarian Syndrome (PCOS). This work was designed to study the implication of IL10 gene polymorphisms (−1082 G/A and −819 C/T) on the susceptibility of Egyptian women to have PCOS. Rotterdam consensus criteria were used to diagnose PCOS patients. Genotyping was performed by single-stranded polymorphism-polymerase chain reaction (SSP-PCR) in 61 PCOS patients and 80 healthy controls, and IL-10 serum levels were measured using Enzyme linked immunosorbent assay (ELISA). The frequency of IL10 −1082 G/G (46%) genotype was significantly increased (p<0.001) while the frequency of −1082 A/A (16%) genotype was significantly decreased (p<0.05) in PCOS patients compared to controls (14% and 35% for G/G and A/A genotypes; respectively). G allele (65%) is significantly increased (p<0.01( in PCOS patients while A allele (61%) is significantly increased (p<0.001( in control subjects. The distribution of IL10 -819 T/T genotype was significantly increased (p<0.05) in PCOS group. G/G genotype (odd ratio (OR=5.322) with confidence interval (CI=2.364–11.982) and the G allele (OR=2.828 with CI=1.73–4.61) of −1082 G/A and T/T genotype of −819 C/T (OR=4.18 with CI=1.26–13.86) could be considered as risk factors for PCOS. IL-10 levels were significantly lower among PCOS patients (313.42±30.10) compared to normal controls (4914.36±303.72). Depending on our preliminary work, IL10 −1082 G/G might be considered as a host genetic factor for PCOS susceptibility in Egyptian women. Studies concerning other cytokine gene polymorphisms are required to get a better understanding of the pathogenesis of PCOS disease.
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