Interleukin 10 (− 1082 G/A) and (− 819 C/T) gene polymorphisms in Egyptian women with polycystic ovary syndrome (PCOS)

Abstract

Cytokines play critical roles in the pathogenesis of Polycystic Ovarian Syndrome (PCOS). This work was designed to study the implication of IL10 gene polymorphisms (−1082 G/A and −819 C/T) on the susceptibility of Egyptian women to have PCOS. Rotterdam consensus criteria were used to diagnose PCOS patients. Genotyping was performed by single-stranded polymorphism-polymerase chain reaction (SSP-PCR) in 61 PCOS patients and 80 healthy controls, and IL-10 serum levels were measured using Enzyme linked immunosorbent assay (ELISA). The frequency of IL10 −1082 G/G (46%) genotype was significantly increased (p<0.001) while the frequency of −1082 A/A (16%) genotype was significantly decreased (p<0.05) in PCOS patients compared to controls (14% and 35% for G/G and A/A genotypes; respectively). G allele (65%) is significantly increased (p<0.01( in PCOS patients while A allele (61%) is significantly increased (p<0.001( in control subjects. The distribution of IL10 -819 T/T genotype was significantly increased (p<0.05) in PCOS group. G/G genotype (odd ratio (OR=5.322) with confidence interval (CI=2.364–11.982) and the G allele (OR=2.828 with CI=1.73–4.61) of −1082 G/A and T/T genotype of −819 C/T (OR=4.18 with CI=1.26–13.86) could be considered as risk factors for PCOS. IL-10 levels were significantly lower among PCOS patients (313.42±30.10) compared to normal controls (4914.36±303.72). Depending on our preliminary work, IL10 −1082 G/G might be considered as a host genetic factor for PCOS susceptibility in Egyptian women. Studies concerning other cytokine gene polymorphisms are required to get a better understanding of the pathogenesis of PCOS disease.