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Abstract
A cDNA that codes for human rheumatoid synovial fluid phospholipase A 2 (PLA 2) hybridised with a RNA of the same size (900 base pairs) isolated from rabbit articular chondrocytes (RAC). Treatment of RAC with 100 ng/ml recombinant human interleukin-1β (IL-1) for 24 hours caused a 13-fold increase in mRNA for this PLA 2. Timecourse studies demonstrated that maximal induction of PLA 2 mRNA occurred by 16 hours post addition of IL-1 (100 ng/ml). Augmentation of RAC PLA 2 mRNA levels was dose-dependent; half-maximal induction was estimated to require 0.15 ng/ml IL-1. Actinomycin D inhibited IL-1 effects on PLA 2 mRNA levels. Coordinate effects of IL-1 on RAC PLA 2 activity were observed with respect to time and dose dependence as well as actinomycin D sensitivity.
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